Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham

A. Jofré; B. Martin; M. Garriga; M. Hugas; M. Pla; D. Rodríguez-Lázaro; T. Aymerich

doi:10.1016/j.fm.2004.04.009

Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham

A. Jofré
, B. Martin
, M. Garriga
, M. Hugas
, M. Pla
, D. Rodríguez-Lázaro
, T. Aymerich

Granja Camps I Armet, E-17121 M.

Research output: Contribution to journal › Scientific article › peer-review

108 Citations (Scopus)

Abstract

A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes, respectively. To ensure the detection of the pathogens in cooked ham, samples were enriched in both buffered peptone-water and Half Fraser broth. Subsequently, equal volumes of enrichment broths were mixed and DNA purification was performed prior to m-PCR reaction, saving considerable time and effort. The m-PCR also proved to be very useful as a simple and ready-to-go method for simultaneous confirmation of presumptive L. monocytogenes and Salmonella spp. colonies directly from agar plates without any DNA extraction steps.

Original language	English
Pages (from-to)	109-115
Number of pages	7
Journal	Food Microbiology
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/j.fm.2004.04.009
Publication status	Published - Jan 2005

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title = "Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham",

abstract = "A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes, respectively. To ensure the detection of the pathogens in cooked ham, samples were enriched in both buffered peptone-water and Half Fraser broth. Subsequently, equal volumes of enrichment broths were mixed and DNA purification was performed prior to m-PCR reaction, saving considerable time and effort. The m-PCR also proved to be very useful as a simple and ready-to-go method for simultaneous confirmation of presumptive L. monocytogenes and Salmonella spp. colonies directly from agar plates without any DNA extraction steps.",

author = "A. Jofr{\'e} and B. Martin and M. Garriga and M. Hugas and M. Pla and D. Rodr{\'i}guez-L{\'a}zaro and T. Aymerich",

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T1 - Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham

AU - Jofré, A.

AU - Martin, B.

AU - Garriga, M.

AU - Hugas, M.

AU - Pla, M.

AU - Rodríguez-Lázaro, D.

AU - Aymerich, T.

PY - 2005/1

Y1 - 2005/1

N2 - A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes, respectively. To ensure the detection of the pathogens in cooked ham, samples were enriched in both buffered peptone-water and Half Fraser broth. Subsequently, equal volumes of enrichment broths were mixed and DNA purification was performed prior to m-PCR reaction, saving considerable time and effort. The m-PCR also proved to be very useful as a simple and ready-to-go method for simultaneous confirmation of presumptive L. monocytogenes and Salmonella spp. colonies directly from agar plates without any DNA extraction steps.

AB - A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes, respectively. To ensure the detection of the pathogens in cooked ham, samples were enriched in both buffered peptone-water and Half Fraser broth. Subsequently, equal volumes of enrichment broths were mixed and DNA purification was performed prior to m-PCR reaction, saving considerable time and effort. The m-PCR also proved to be very useful as a simple and ready-to-go method for simultaneous confirmation of presumptive L. monocytogenes and Salmonella spp. colonies directly from agar plates without any DNA extraction steps.

UR - https://www.scopus.com/pages/publications/4344710719

U2 - 10.1016/j.fm.2004.04.009

DO - 10.1016/j.fm.2004.04.009

M3 - Scientific article

AN - SCOPUS:4344710719

SN - 0740-0020

VL - 22

SP - 109

EP - 115

JO - Food Microbiology

JF - Food Microbiology

IS - 1

ER -

Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham

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