Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

David Rodríguez-Lázaro; Anna Jofré; Teresa Aymerich; Marta Hugas; Maria Pla

doi:10.1128/AEM.70.10.6299-6301.2004

Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

David Rodríguez-Lázaro
, Anna Jofré
, Teresa Aymerich
, Marta Hugas
, Maria Pla

Institut de Recerca I Tecnología Agroalimentaries (IRTA)
European Food Safety Authority

Research output: Contribution to journal › Scientific article › peer-review

88 Citations (Scopus)

Abstract

We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

Original language	English
Pages (from-to)	6299-6301
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	70
Issue number	10
DOIs	https://doi.org/10.1128/AEM.70.10.6299-6301.2004
Publication status	Published - Oct 2004

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10.1128/AEM.70.10.6299-6301.2004

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abstract = "We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.",

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AU - Rodríguez-Lázaro, David

AU - Jofré, Anna

AU - Aymerich, Teresa

AU - Hugas, Marta

AU - Pla, Maria

PY - 2004/10

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N2 - We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

AB - We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

UR - https://www.scopus.com/pages/publications/8144221942

U2 - 10.1128/AEM.70.10.6299-6301.2004

DO - 10.1128/AEM.70.10.6299-6301.2004

M3 - Scientific article

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JO - Applied and Environmental Microbiology

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Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

Abstract

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