Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

David Rodríguez-Lázaro; Anna Jofré; Teresa Aymerich; Marta Hugas; Maria Pla

doi:10.1128/AEM.70.10.6299-6301.2004

Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

David Rodríguez-Lázaro
, Anna Jofré
, Teresa Aymerich
, Marta Hugas
, Maria Pla

Institut de Recerca I Tecnología Agroalimentaries (IRTA)
European Food Safety Authority

Producció científica: Contribució a revista › Article científic › Avaluat per experts

88 Cites (Scopus)

Resum

We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

Idioma original	Anglès
Pàgines (de-a)	6299-6301
Nombre de pàgines	3
Revista	Applied and Environmental Microbiology
Volum	70
Número	10
DOIs	https://doi.org/10.1128/AEM.70.10.6299-6301.2004
Estat de la publicació	Data de publicació - d’oct. 2004

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10.1128/AEM.70.10.6299-6301.2004

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title = "Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR",

abstract = "We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.",

author = "David Rodr{\'i}guez-L{\'a}zaro and Anna Jofr{\'e} and Teresa Aymerich and Marta Hugas and Maria Pla",

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T1 - Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

AU - Rodríguez-Lázaro, David

AU - Jofré, Anna

AU - Aymerich, Teresa

AU - Hugas, Marta

AU - Pla, Maria

PY - 2004/10

Y1 - 2004/10

N2 - We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

AB - We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

UR - https://www.scopus.com/pages/publications/8144221942

U2 - 10.1128/AEM.70.10.6299-6301.2004

DO - 10.1128/AEM.70.10.6299-6301.2004

M3 - Scientific article

C2 - 15466579

AN - SCOPUS:8144221942

SN - 0099-2240

VL - 70

SP - 6299

EP - 6301

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR

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